Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Alkali Scientific SmartReader™ 96 Microplate Absorbance Reader, for 96 Well Plates, 115V QTY 1
The Accuris SmartReader 96 is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 well plates. The instrument’s 7 inch touch screen, intuitive software and graphical interface makes it easy to use as a standalone instrument (a separate computer and additional software is not required).
Molecular Devices SpectraMax i3x Microplate Reader, Multi-Mode, 6-1536 Well
The SpectraMax i3x is a configurable multi-mode microplate reader designed for biochemical and cell-based assays. It provides absorbance, fluorescence, and luminescence detection across a broad wavelength range, supports 6-1536 well plates, and can be field-upgraded with imaging, TRF/HTRF, AlphaScreen, injectors, and western blot detection modules.
- Configurable multi-mode detection for absorbance, fluorescence, and luminescence.
- Supports plate formats from 6 to 1536 wells.
- Broad wavelength coverage from UV to near-infrared.
- Field-upgradeable modules for imaging, TRF/HTRF, AlphaScreen, injectors, and western blot detection.
- High-sensitivity optics with xenon flash lamp and LED illumination options.
- Compact footprint suitable for bench or automated workflows.
Molecular Devices Fluorescence Emission Filter, 550 nm, 10 nm
Emission filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), intended for selective fluorescence detection in multi-mode microplate readers that accept standard filter slides or mounts.
- Center wavelength 550 nm.
- Bandwidth 10 nm (FWHM).
- Emission bandpass design for fluorescence detection.
- Reduces spectral crosstalk and improves signal specificity.
- Suitable for use in multi-mode microplate readers and compatible filter slide systems.
Molecular Devices Filter Lens, 550 nm, EMax and VMax Compatible
Replacement optical filter lens with a center wavelength of 550 nm for use in microplate readers. Designed as a field-replaceable spare to restore or modify detection for assays that require a 550 nm bandpass. Supplied as a single unit for straightforward replacement.
- Center wavelength 550 nm.
- Compatible with EMax and VMax microplate readers.
- Single replacement filter, sold each.
- Suitable for absorbance and fluorescence measurements requiring a 550 nm bandpass.
Molecular Devices Fluorescence Polarization Filter, Polarized Horizontal, 400-750 nm
The fluorescence polarization filter is an accessory optical filter designed for polarized fluorescence measurements on compatible microplate readers. It provides a horizontal polarization orientation and covers broad visible wavelengths; the filter is supplied with a holder for installation in the instrument's filter cartridge or slide system.
- Polarized horizontal orientation for fluorescence polarization assays.
- Covers 400 nm-750 nm wavelength range.
- Supplied with a holder for installation in the filter cartridge or slide.
- Designed for use in compatible microplate readers to enable polarized fluorescence detection.
Molecular Devices Filter Lens, 492 nm Bandpass, Microplate Reader Compatible
The Molecular Devices 492 nm filter lens (MPN 6500-0048) is a replacement optical element that isolates the 492 nm detection band for multimode microplate readers. It is intended for assays requiring targeted excitation or detection at this wavelength and for use as a service spare for compatible readers.
- Provides narrowband filtration centered at 492 nm for targeted detection.
- Supports absorbance and fluorescence assay modalities.
- Serves as a replacement optical element for compatible multimode microplate readers.
- Facilitates reliable wavelength selection for plate-based measurements.
- Intended for use as an OEM spare component in instrument service and maintenance.
Molecular Devices Fluorescence Emission Filter, 660 nm, 32 nm Bandwidth
This emission filter is centered at 660 nm with a 32 nm full bandwidth and is intended for use in filter-based fluorescence microplate readers to isolate red emission wavelengths for assays using long-wavelength fluorophores.
- Center wavelength: 660 nm.
- Bandwidth (full width at half maximum): 32 nm.
- Product type: fluorescence emission filter for microplate readers.
- Application: isolates red emission for assays using red fluorophores and dyes.
- Benefit: improves spectral selectivity and reduces signal crosstalk between channels.
Revvity Health Sciences Inc HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human VHL Binding Kit, 100,000 Assay Points
HTRF Human VHL Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Ataxin 2 Detection Kit, 500 Assay Points
HTRF Human & Mouse Ataxin 2 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points
HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points