Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Molecular Devices 370 nm time-resolved fluorescence filter provides excitation at 370 nm for time-resolved fluorescence assays. It is supplied as a single replacement optical filter for compatible fluorescence instruments.
- Provides excitation centered at 370 nm.
- Designed for time-resolved fluorescence measurements.
- Supplied as a single replacement optical filter.
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- Helps improve excitation specificity for time-resolved assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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This excitation filter provides a 535 nm center wavelength with a 25 nm full-width bandwidth for fluorescence excitation in microplate assays. It is intended for use with multi-mode fluorescence microplate readers to enable selective excitation of fluorophores around 535 nm.
- Center wavelength 535 nm.
- Bandwidth 25 nm full width.
- Excitation filter for fluorescence detection.
- Compatible with multi-mode microplate and ELISA readers.
- Designed for microplate-based fluorescence assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices Low-Volume Slide Refill Kit, 64-Well, Pack of 3
Replenishment kit of low-volume bottom slides for slide-based microplate readers. The kit includes three low-volume bottom slides, each with 64 wells, intended for low-volume absorbance and related assays.
- Contains three 64-well low-volume bottom slides.
- Intended for low-volume absorbance and slide-based assays.
- Supplies replacement slides to restore slide reader capacity.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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For use with EMS SmartDoc gel imaging system.
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