Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF CHO HCP Detection Kit, 10,000 Assay Points
HTRF CHO HCP Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total MerTK Detection Kit, 500 Assay Points
HTRF Human Total MerTK Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, Control Lysate
HTRF Human Total PR Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse TOM20 Detection Kit Standard
HTRF Human & Mouse TOM20 Detection Kit Standard
Revvity Health Sciences Inc HTRF Human & Mouse Total NRF2 Detection Kit, Control Lysate
HTRF Human & Mouse Total NRF2 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human IL12/23 p40 Detection Kit, Standard, 1 Vial
HTRF Human IL12/23 p40 Detection Kit, Standard, 1 Vial
Revvity Health Sciences Inc HTRF Human Total BRD3 Detection Kit, 500 Assay Points
HTRF Human Total BRD3 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF AAV1 Detection Kit, 10,000 Assay Points
HTRF AAV1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF AAV5 Detection Kit, 10,000 Assay Points
HTRF AAV5 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Cyclin A2 Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total Cyclin A2 Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human Total Cyclin E1 Detection Kit, 10,000 Assay Points
HTRF Human Total Cyclin E1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA Human 7-36 amide GLP1 Detection Kit, 100 Assay Points
AlphaLISA Human 7-36 amide GLP1 Detection Kit, 100 Assay Points
Molecular Devices Fluorescence Filter, 447 nm, 60 nm, With Holder
Fluorescence donor emission filter for SpectraMax iD5 and iD5e multi-mode microplate readers, centered at 447 nm with a 60 nm bandwidth, supplied with a holder for mounting and optical alignment.
- Isolates emission centered at 447 nm with a 60 nm bandwidth.
- Includes holder for secure mounting in the instrument filter module.
- Compatible with fluorescence and NanoBRET assays on multi-mode microplate readers.
- Improves signal separation for donor emission detection and assay sensitivity.
- Designed for easy installation and reliable optical alignment.
Molecular Devices Emission Filter, 585 nm Center, 10 nm Bandwidth
Optical emission bandpass filter centered at 585 nm with a 10 nm full width at half maximum (FWHM). Designed for fluorescence assays in filter-based microplate readers, the filter isolates emission signals to improve signal-to-noise and measurement accuracy when used in compatible filter holders.
- Center wavelength 585 nm.
- Bandwidth 10 nm FWHM.
- Emission bandpass optical filter for fluorescence detection.
- Reduces background and improves signal-to-noise ratio.
- Fits standard filter holders for compatible multi-mode microplate readers.
- Suitable for plate-based fluorescence assays with emission near 585 nm.
Molecular Devices Excitation Filter, 485 nm, 20 nm Bandwidth
Excitation filter for fluorescence microplate assays centered at 485 nm with a 20 nm bandwidth. The filter is designed for installation in filter slide holders and is used to selectively excite blue-range fluorophores in fluorescence intensity and ELISA workflows.
- Precise 485 nm center wavelength for blue-excited fluorophores.
- 20 nm bandwidth for selective excitation and reduced background signal.
- Designed for installation in filter slide holders used by multi-mode microplate readers.
- Suitable for fluorescence intensity assays and ELISA workflows.
- Replaceable optical element for routine maintenance and assay optimization.