Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Fluorescence Filter, 700 nm, 60 nm Bandwidth, Holder
Molecular Devices fluorescence emission filter with holder (MPN 6590-0114) provides selective far-red detection at a 700 nm center wavelength with a 60 nm optical bandwidth. It is sold as a replaceable accessory for compatible multi-mode microplate readers and mounts in the instrument's filter holder for reliable signal selection.
- Center wavelength: 700 nm.
- Bandwidth: 60 nm.
- Filter type: emission filter for far-red fluorescence.
- Includes holder for instrument mounting.
- Typical application: far-red fluorescence assays on compatible readers.
Ace Glass, Inc. Stir motor, DC, 1/40hp, dual shaft, reversible, w/o mounting bracket, CE & UL rated
Stir motor, DC, 1/40hp, dual shaft, reversible, w/o mounting bracket, CE & UL rated
Molecular Devices Fluorescence Filter, 447 nm, 60 nm, With Holder
Fluorescence donor emission filter for SpectraMax iD5 and iD5e multi-mode microplate readers, centered at 447 nm with a 60 nm bandwidth, supplied with a holder for mounting and optical alignment.
- Isolates emission centered at 447 nm with a 60 nm bandwidth.
- Includes holder for secure mounting in the instrument filter module.
- Compatible with fluorescence and NanoBRET assays on multi-mode microplate readers.
- Improves signal separation for donor emission detection and assay sensitivity.
- Designed for easy installation and reliable optical alignment.
Molecular Devices Emission Filter, 650 nm, 40 nm, FilterMax
Replacement fluorescence emission filter with a 650 nm center wavelength and 40 nm bandwidth, designed for use in Molecular Devices FilterMax F-series multi-mode microplate readers to collect far-red fluorescence emission.
- Center wavelength 650 nm.
- Bandwidth 40 nm for selective far-red detection.
- Designed for installation in filter slide assemblies.
- Enables assays requiring far-red emission collection with reduced background.
- Replacement part for multi-mode microplate reader fluorescence modules.
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Molecular Devices Absorbance Filter, 575 nm, 8 nm Bandwidth
This absorbance filter provides narrowband detection at a 575 nm center wavelength with an 8 nm full-width bandwidth for visible-range absorbance assays. It is designed for use in FilterMax F3 and F5 multi-mode microplate readers.
- Provides narrowband absorbance detection at 575 nm.
- 8 nm full-width bandwidth for selective wavelength measurement.
- Designed for use with FilterMax F3 and F5 multi-mode microplate readers.
- Suitable for visible-range absorbance assays and endpoint measurements.
Molecular Devices Fluorescence Excitation Filter, 550 nm, 10 nm
A narrow-band fluorescence excitation filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), designed for microplate fluorescence assays to isolate excitation light and improve signal specificity.
- Provides narrow-band excitation centered at 550 nm.
- 10 nm full width at half maximum for selective excitation.
- Optimized for microplate fluorescence measurements.
- Reduces background and improves signal-to-noise ratio.
- Designed for use in compatible microplate fluorescence readers.
Molecular Devices Emission Filter, 574 nm, 25 nm BW
Fluorescence emission filter centered at 574 nm with a 25 nm bandwidth, designed for fluorescence detection in microplate and ELISA readers.
- Center wavelength 574 nm.
- Bandwidth 25 nm.
- Suitable for fluorescence detection in microplate and ELISA readers.
- Sold as a single unit.
- Country of origin US.
Molecular Devices Fluorescence Filter Kit, EM 660 nm, 40 nm
The SpectraMax Fluorescence Intensity Filter Kit delivers five exchangeable optical filters for fluorescence, luminescence, and time-resolved fluorescence (TRF) assays on compatible multi-mode microplate readers. The kit includes an emission 660 nm filter with a 40 nm bandwidth plus additional excitation and emission filters to support common assays.
- Includes five exchangeable filters covering common fluorescence wavelengths from ~400-700 nm.
- Contains an emission 660 nm filter with a 40 nm full bandwidth for near-IR fluorescence detection.
- Supports fluorescence intensity, luminescence, and time-resolved fluorescence assay modes.
- Allows quick filter exchange to adapt to different assay requirements.
- Designed for use with compatible multi-mode microplate readers to expand detection flexibility.
Molecular Devices Fluorescence Polarization Filter, 595 nm, 35 nm, Horizontal
This fluorescence polarization filter is specified for fluorescence polarization assays on compatible microplate readers. It has a 595 nm center wavelength with a 35 nm bandwidth and a polarized horizontal orientation, and is supplied with a holder for mounting in standard filter slides or modules.
- Center wavelength 595 nm.
- Bandwidth 35 nm.
- Polarization: horizontal.
- Includes holder for filter slide or module mounting.
- Intended for fluorescence polarization assays on compatible multi-mode microplate readers.
Molecular Devices Emission Slide, Empty, Slide 21, FilterMax Compatible
The empty emission slide is an unpopulated emission filter slide designed to accept user-selected emission filters for custom fluorescence configurations on compatible microplate readers. It installs into the reader's filter slider assembly to provide flexible emission filter selection for fluorescence assays while excluding fluorescence polarization methods.
- Empty emission filter slide for custom filter installation.
- Accepts emission filter elements for fluorescence detection.
- Configurable for fluorescence methods except fluorescence polarization.
- Supplied as a single slide for in-lab or service installation.
- Installs into the reader filter slider assembly for filter mounting.